中国科学院植物研究所系统与进化植物学国家重点实验室

中国珍稀濒危植物DNA条形码鉴定平台

DNA Barcode of Rare and Endangered Plant (BREP)

最新进展

应用案例

科研项目

研究团队

图片欣赏

联系我们

Discriminating plants using the DNA barcode rbcLb: An appraisal based on a large dataset

Wenpan Dong, Tao Cheng, Changhao LI, Chao Xu, Ping Long, Chumming Chen, Shiliang Zhou

The ideal DNA barcode for plants remains to be discovered, and the candidate barcode rbcL has been met with considerable skepticism since its proposal. In fact, the variability within this gene has never been fully explored across all plant groups from algae to flowering plants, and its performance as a barcode has not been adequately tested.
By analyzing all of the rbcL sequences currently available in GenBank, we attempted to determine how well a region of rbcL performs as a barcode in species discrimination. We found that the rbcLb region was more variable than the frequently used rbcLa region. Both universal and plant group-specific primers were designed to amplify rbcLb, and the performance of rbcLa and rbcLb was tested in several ways. Using BLAST, both regions successfully identified all families and nearly all genera; however, the successful species identification rates varied significantly among plant groups, ranging from 24.58% to 85.50% for rbcLa and from 36.67% to 90.89% for rbcLb. Successful species discrimination ranged from 5.19% to 96.33% for rbcLa and from 22.09% to 98.43% for rbcLb in species-rich families and from 0 to 88.73% for rbcLa and from 2.04% to 100% for rbcLb in species-rich genera. Both regions performed better for lower plants than for higher plants, though rbcLb performed significantly better than rbcLa overall, particularly for angiosperms.
Considering the applicability across plants, easy and unambiguous alignment, high primer universality, high sequence quality, and high species-discrimination power for lower plants, we suggest rbcLb as a universal plant barcode.

002

返回

系统与进化植物学国家重点实验室 中国科学院植物研究所 地址:北京市海淀区香山南辛村20号

联系电话:(010)62836503 邮编:100093 邮箱:brep@ibcas.ac.cn 京ICP备13049593号